siRNA synthesis has been globally recognized as one of the most effective ways for the silencing of target genes in cells or body, and is widely accepted due to its low cost and high transfection efficiency.
The well-known siRNA synthesis is not only the optimum method for RNAi‐based clinical treatment, but also irreplaceable in drug development.
In collaboration with Cohesion Biosciences (http://www.cohesionbio.com/), Bio Basic Asia Pacific now provides two types of siRNA products, standard siRNA and chemically-modified siRNA, with various modifications of siRNA available in accordance to our customer’s requirements.
1. Standard siRNA product
The single/unpaired strands in standard siRNA products provided by us have been completely removed via HPLC method. They can be used directly in experiments after dilution with the buffer provided. The standard siRNA products directly target the mRNA of interest; therefore, it is recommended to detect the expression level of the target mRNA by real-time PCR to ensure the effectiveness of siRNA products.
2. Chemically modified siRNA
Bio Basic Asia Pacific provides a wide variety of chemically modified siRNA products, such as 2’-hydroxymethylation modification, Phosphorothioate modification, Fluoro modification, etc.. With advanced technology for siRNA synthesis, our production team has addressed the concern of siRNA stability in transfection experiments via chemical modification.
To enhance the siRNA stability, the four ends of the double stranded siRNA were labeled with fluorescent markers. As compared to unlabeled siRNA, the labeled siRNA shows the advantage of high visibility during transfection process. The labeled siRNA can be easily observed by the use of flow cytometry, fluorescence microscope, laser confocal microscope and so on. This leading technology is not only advantageous in optimizing the transfection conditions and intracellular localization of siRNA, but also in tracing and analyzing the relationship between the transfection efficiency and protein expression during the whole transfection process.
Terminal Modification | Base Modification |
5′ FAM | 2′ Fluoro dU |
5′ Biotin | 2′ Fluoro dC |
5′ Amine | 2′ OMe rU |
5′ Phosphate | 2′ OMe rC |
5′ Thiol | 2′ OMe rG |
5′ Cholesterol | 2′ OMe rA |
Product Information | Final Yield | Purification | Turnaround/Lead Time |
Standard siRNA | 2 – 250 OD | HPLC | 6 – 15 Working Days |
Chemically-modified siRNA | 5 – 250 OD |
*Price (SGD): Pricing may vary depending on desired final yield and purification method
For ordering or request of quote, kindly provide us the following information and email to sg@biobasic-asia.com:
Deliverables
Upon the completion of project, we will provide:
Storage and Stability
Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, recommended storage condition should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. Storing at concentrations above 20uM is recommended. We guaranteed the quality of oligonucleotides for 6 months upon receipt, if stored under the above conditions.