Oligo Modifications List

Internal Modification

5’ Modification

3’ Modification

5’ and 3’ Modification

5-Hydroxmethyl dC

5′ P

3′ P

5′ 6-FAM, 3′ BHQ1

5-Nitroindole

5′ NH2 C6

3′ NH2 C6

5′ 6-FAM, 3′ BHQ2

Uni-link Amino

5′ NH2 C12

3′ NH2 C7

5′ 6-FAM, 3′ TAMRA

N6-Me-dA

5′ Biotin

3′ Biotin

5′ 6-FAM, 3′ Dabcyl

Azobenzene

5′ Biotin-TEG

3′ Biotin-TEG

5′ 6-FAM, 3′ Eclipse

Phosphorothioate

5′ Desthio Biotin-TEG

3′ Desthio Biotin-TEG

5′ 6-FAM, 3′ MGB

DeoxyInosine

5′ Dual Biotin

3′ SH C3

5′ TET, 3′ BHQ1

DeoxyUridine

5′ triple Biotin

3′ SH C6

5′ TET, 3′ BHQ2

XNA

5′ SH C6

3′ Dig

5′ TET, 3′ TAMRA

RNA

5′ Dithiol

3′ Inverted dT

5′ TET, 3′ Dabcyl

2F-RNA

5′ triple SH

3′ ddC

5′ TET, 3′ MGB

2′ -O-Methyl

5′ Dig

3′ AMCA

5′ JOE, 3′ BHQ1

5-Methyl dC

5′ AMCA

3′ Alexa Fluor 488

5′ JOE, 3′ BHQ2

2-Aminopurine

5′ Alexa Fluor 488

3′ 6-FAM

5′ JOE, 3′ TAMRA

5-Bromo dU

5′ 6-FAM

3′ TET

5′ JOE, 3′ Dabcyl

8-OXO-dG

5′ TET

3′ JOE

5′ JOE, 3′ MGB

C3 Spacer

5′ JOE

3′ HEX

5′ HEX, 3′ BHQ1

C6 Spacer

5′ HEX

3′ Cy3

5′ HEX, 3′ BHQ2

C12 Spacer

5′ VIC

3′ TAMRA

5′ HEX, 3′ TAMRA

Spacer 9

5′ Cy3

3′ ROX

5′ HEX, 3′ Dabcyl

Spacer 18

5′ TAMRA

3′ Texas Red

5′ HEX, 3′ Eclipse

dSpacer

5′ NED

3′ Quasar 670

5′ HEX, 3′ MGB

1-Me-dA

5′ ROX

3′ Cy5

5′ VIC, 3′ BHQ1

PC-Linker

5′ Texas Red

3′ CY5.5

5′ VIC, 3′ BHQ2

Pyrrolo-dC

5′ Quasar 670

3′ Atto 425

5′ VIC, 3′ MGB

Inverted dT

5′ Cy5

3′ Atto 590

5′ CY3, 3′ BHQ2

5-Aza-2′ –deoxycytidine

5′ CY5.5

3′ AquaPhluor 593

5′ CY3, 3′ Dabcyl

6-FAM-dT

5′ Cy7

3′ Yakima Yellow

5′ CY3, 3′ MGB

HEX dT

5′ Atto 425

3′ Methylene Blue

5′ TAMRA, 3′ BHQ2

ROX-dT

5′ Atto 590

3′ Maleimide

5′ TAMRA, 3′ Dabcyl

TAMRA-dT

5′ Methylene Blue

3′ Ru

5′ TAMRA, 3′ Eclipse

TET-dT

5′ Maleimide

3′ BHQ1

5′ TAMRA, 3′ MGB

Texas Red dT

5′ BHQ1

3′ BHQ2

5′ ROX, 3′ BHQ2

Cy5 dT

5′ BHQ2

3′ BHQ3

5′ ROX, 3′ Dabcyl

Cy3

5′ Dabcyl

3′ MGB

5′ ROX, 3′ Eclipse

Cy3 dT

5′ Ferrocene

3′ Dabcyl

5′ ROX, 3′ MGB

BHQ1 dT

5′ Acrydite

3′ Eclipse

5′ Texas Red, 3′ BHQ2

BHQ2 dT

5′ Azide (N3)

3′ Ferrocene

5′ Texas Red, 3′ Dabcyl

NH2 C6 dT

5′ Ru

3′ Azide (N3)

5′ Texas Red, 3′ MGB

Biotin dT

5′ CHO

3′ CHCH

5′ Quasar 670, 3′ BHQ2

Dabcyl dT

5′ CHCH

3′ Cholesteryl

5′ Quasar 670, 3′ BHQ3

Dig dT

5′ COOH

3′ dT-Q

5′ CY5, 3′ BHQ2

Dithiol

5′ Symmetric

3′ dG-Q

5′ CY5, 3′ BHQ3

HS-SH C6

5′ DBCO

3′ DBCO

5′ CY5, 3′ Dabcyl

Methylene Blue dT

5′ Desthio Biotin

3′ Dithiol

5′ CY5, 3′ MGB

Azide dT

5`Pyrene

3′ Desthio Biotin

5′ CY5.5, 3′ BHQ2

CHCH dT

DBCO dT

Symmetric

Ferrocene dT

Alexa Fluor 488-dT

JOE-dT