Iso-Sequencing on PacBio

Changes in isoform variants and relative abundancies are an important clue in deciphering how genomic variants drive the phenotypic differences between health and disease. Only long-read sequencing can provide unbiased, direct detection of all the isoforms present in the samples.

Iso-sequencing analysis allows us to generate full length cDNA sequences without assembly. It characterizes transcript isoforms within targeted genes or across an entire transcriptome.

Sequencing Platforms

PacBio Sequel

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Bioinformatics Analysis

  • Data quality control
  • Annotation of gene Function, CDS prediction and SSR detection
  • Expression and differential expression
  • GO and KEGG
(More analysis upon request)


  • Discover new genes, transcripts, and alternative splicing events
  • Improve genome annotation to identify gene structure, regulatory elements, and coding regions
  • Increase the accuracy of RNA-sequencing quantification with isoform-level resolution

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