Iso-Sequencing on PacBio
Changes in isoform variants and relative abundancies are an important clue in deciphering how genomic variants drive the phenotypic differences between health and disease. Only long-read sequencing can provide unbiased, direct detection of all the isoforms present in the samples.
Iso-sequencing analysis allows us to generate full length cDNA sequences without assembly. It characterizes transcript isoforms within targeted genes or across an entire transcriptome.
Sequencing Platforms
PacBio Sequel
For more information and quotation, kindly contact us at NGS@biobasic-asia.com
Bioinformatics Analysis
- Data quality control
- Annotation of gene Function, CDS prediction and SSR detection
- Expression and differential expression
- GO and KEGG
Advantages
- Discover new genes, transcripts, and alternative splicing events
- Improve genome annotation to identify gene structure, regulatory elements, and coding regions
- Increase the accuracy of RNA-sequencing quantification with isoform-level resolution
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