Welcome to Bio Basic Asia Pacific Pte Ltd
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Our Experience in DNA Sequencing
DNA sequencing and Sanger sequencing at Bio Basic Asia Pacific provide researchers with over 10 years of specialized expertise in delivering high-accuracy genetic analysis. Our Sanger DNA sequencing service utilizes advanced capillary electrophoresis of fluorescent-labelled DNA fragments to achieve consistent read lengths of 800–1000bp, ensuring maximum data yield for every reaction.
DNA Sequencing Workflow
DNA Sequencing Service Types
i) Standard Sequencing (Steps 1 – 8)
ii) *Enzymatic Clean-up (Default) / Magnetic beads (Select this at Step 4 if required)
iii) *Gel Extraction (Select this at Step 4 if required)
iv) #Ready to run with dye removal (Select this if Steps up to (5) has been pre-performed by customer)
v) #Ready to run (Dry Pellet) (Select this if Steps up to (6) has been pre-performed by customer)
Accepted Sample Types
Purified PCR products
Raw PCR products
Plasmids Samples
RCA Products
Free Additional Runs
A one time free re-sequencing is available upon request. Kindly email us at sg-sequencing@biobasic-asia.com with your request and the team will assist you accordingly.
Sometimes better results are obtained in the second run whereby further optimization is performed as possible issues discovered during the initial run allows for the team to use a more suitable protocol for the DNA sequencing.
Hence, it is important to provide us with information such as presence of hairpin loop in the sequencing template, GC-rich region, or sample with low concentration etc, so that the team can take note and adopt an appropriate protocol in the first run.
Free Gel Analysis
We will perform gel analysis at no additional cost for QC of the fragment length.
Gel QC will be sent along with the Sequencing results.
100+ Free Universal Primers
We provide more than 100 free universal primers to cater to your sequencing reaction.
Kindly find the downloadable primer list with their respective sequence here.
Short Turnaround Time
Within 24 hours upon sample receival on a normal working day. This applies to orders received on a Friday as well.
- Additional 1 working day required for Gel Extraction and Magnetic Beads services (Locally based Customers)
1) Enzymatic Clean-up (Default)
Enzymatic cleanup of crude PCR samples using ExoSAP:
- Recombinant, Heat Labile Shrimp Alkaline Phosphatase: removes excess nucleotides via dephosphorylation.
- Recombinant, Heat Labile Exonuclease I: Degrades single stranded DNA such as excess oligonucleotide primers.
2) Magnetic Beads
Magnetic beads bind reversibly to nucleic acids and are immobilised through an external magnetic field, allowing the removal of impurities during the wash step. After which, purified DNA samples are released from the magnetic beads with the use of an elution buffer and the removal of the external magnetic field.
3) Gel Extraction
Gel extraction enables the removal of impurities such as nucleotides enzymes, salts etc via direct isolation of desired DNA fragments of interest.
1) Ready to run with dye removal
Cycle sequencing reaction has been performed. Reaction mix after the thermal cycler stage submitted as a sample.
Upon receipt, dye terminator removal will be performed prior processing in the DNA analyzer.
2) Ready to run (Dry Pellet)
Cycle sequencing has been performed and samples have been purified and submitted in dry pellet form.
Upon receipt, the sample will be resuspended for direct processing in the DNA analyzer.
Kindly submit the order via our customer portal site at the top right hand corner of the website.
You may also contact us directly at sg-sequencing@biobasic-asia.com or +65 6954 2519 for any assistance.
Visit our Download page for guide on the ordering process, samples requirements, preparation and other relevant information.
DNA sequencing is the broad service we provide; we specifically use the Sanger sequencing method via capillary electrophoresis to provide highly accurate, long-read genetic data.
Through our optimized protocols, we typically achieve high-quality read lengths between 800bp and 1000bp per reaction.
Yes, we provide over 100 free universal primers to cater to your sequencing reactions, significantly reducing your project costs.
We offer a short 24-hour turnaround time upon sample receipt on normal working days, including orders received on Fridays.
Yes, we perform free gel analysis for QC of fragment length and provide the gel QC images along with your sequencing results.
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