Oligo Synthesis Overview

The quality of our modified oligonucleotides is verified using mass spectrometry to guarantee accuracy and purity. Bio Basic Asia Pacific also provides a diverse range of modifications, allowing researchers to design custom oligos tailored for PCR, cloning, NGS, CRISPR, and other applications.

Our facility maintains a consistent production capacity of 10,000 oligos per day, ensuring high-volume orders can be fulfilled without compromising quality.

• 12-hour turnaround for sequences under 50 bases
• 1–2 business days for sequences 50–120 bases
• Additional 2–3 days for PAGE or HPLC purification
• Turnaround times may vary depending on order volume, complexity, and current production throughput.

We offer multiple purification options to meet research needs:
Desalted, PAGE, HPLC, HPLC-CE
***These methods are available for both unmodified and modified oligonucleotides.

On average it takes about:

• 12-hour turnaround time available for shorter oligos.

• 1-2 business days for longer oligos.
  *Turnaround times will vary on current throughput, volumes and complexity of the order.

• +2-3 days for PAGE or HPLC purification.
  *For more details, please enquire directly.

We can synthesis oligos up to 110 bases in length. Due to manufacturing restrictions we only offer synthesis under PAGE or HPLC purification for oligos ≥60 bases. For our desalted purification method, there is a size restriction for only oligos between 11-59 bases.

We have a large variety of common modifications. You can find our full list on our oligo modifications page. If you cannot find the modification you’re interested in, don’t hesitate to contact us at service@biobasic-asia.com to enquire. Note that some of our modifications may have alternate names as some modification names are proprietary, therefore we are not allowed to list them on our website.

Oligos are lyophilized in microcentrifuge tubes or lyophilized in 96-well plates for automated operations. Note: A minimum of 48 oligos must be ordered for the 96-well Plate format.

To reconstitute: Centrifuge the tube for a few seconds to collect the DNA in the bottom of the tube. Carefully open, add an appropriate volume of buffer of choice, close, re-hydrate for two minutes and vortex for 15 seconds (common buffers are Tris or TE, TE typically used for stability / long term storage). It is recommended for the oligos to be reconstituted to concentrations > 10µM and stored at -20°C.

Storage at -20°C:

The stability of the lyophilized (dry) and reconstituted material (including TE and and nuclease-free water) is approximately 2 years

Storage at at 4°C:

The stability of the lyophilized (dry) and reconstituted material (including TE and and nuclease-free water) is approximately 1 year

Storage at Room Temperature:

The lyophilized (dry) and TE reconstituted oligos are stable for approx. 6 months, while oligos reconstituted in nuclease-free water would be stable for less than 1 month

Note that:

– Oligos are more stable when reconstituted in TE buffer rather than in their lyophilized (dry) form. This is because of the stabilization effect contributed by the Tris (maintains pH) and EDTA (inhibits nucleases)

– Stability varies with modified oligos as the different attachments, linkers, etc. alter the stability of the oligos

– Avoid UV light exposure to minimize photodegradation

At Bio Basic, we allow customers to order via synthesis scale. Based on the individual sequence and purification method, the minimum guaranteed yield may differ from oligo to oligo. As a rule of thumb, we guarantee the following:

*Minimum guaranteed yield for 15-60 bases. For other lengths, please contact the Synthesis Team.

Please click here for our oligo services terms and conditions

For Oligo Orders, kindly provide the following information:

1. A unique oligo name

2. Sequence in 5′ to 3′

3. Desired synthesis scale (in nmol/OD)

4. Desired purification (Desalt, PAGE, HPLC or HPLC-CE)

5. Modifications (if any) in their respective location

For ordering for standard, non-modified oligos, kindly submit the order via our customer portal site.

For purification methods other than “Desalt”, kindly email the completed Oligo Synthesis Order Form to service@biobasic-asia.com to order and request a quote, and we will get back to you as soon as we can.

You may also contact us directly at +65 8208 0600 for any assistance.