Oligonucleotide synthesis is the chemical synthesis of short nucleic acids chains with desired sequence. Oligonucleotides are widely used in molecular biology and medical research in applications such as PCR, cloning, next generation sequencing, CRISPR and other applications. The capability to de novo synthesise nucleic acids from building blocks (2′-deoxynucleosides, ribonucleosides, or chemically modified nucleosides, e.g. Florescence labelled deoxynucleosides) has greatly benefited and expedited research in molecular biology.
Bio Basic Asia Pacific has been consistently synthesizing oligonucleotides (non-modified oligo & modified oligo) for a variety of research applications for over 15 years. Using our in-house reagents and tools, we are able to provide quality & affordable custom oligonucleotides for our customers.
O.D. stands for Optical Density units; it is based on absorbance readings at 260nm. For synthesis facilities that use phosphoramidite synthesis, O.D. measurements are a means to quantify the amount of available oligo after purification i.e. the FINAL YIELD of the oligo. As a rough estimate 1 O.D. ~ 33 ug. When comparing oligo synthesis services, the O.D. should be compared (the final yield) and not the initial synthesis scale (in “nmol”) which is the quantity of the starting material required before synthesis of the oligo. From this, the final “nmol” yield will vary on the length, sequence and molecular weight of the individual oligo.
You can order by “nmol” reference but note that “nmol” is assumed to be the initial amount of material used to synthesize an oligo unless otherwise noted. This is not accurate means to compare oligos between facilities as our manufacturing procedures are likely different and therefore the corresponding final yield and price rates will differ. If you’re switching providers and aren’t quite sure what to pick, don’t hesitate to ask and we will be happy to match an equivalent or better oligo service.
HAP stands for “High Affinity Purification”; it is a patented, novel purification method for custom oligos developed by Bio Basic Asia Pacific. After synthesis the DMT-ON-oligo in the crude oligo mixture is selectively absorbed on a high affinity resin within a HAP column, this allows incomplete oligo fragments pass through while keeping majority of the full length oligos. The full length oligo is retrieved by removing the DMT protection group under mild acid conditions.
The HAP process provides two major advantages, higher purity, superior to that of the De-Salted method (purity of a standard 20 bases-HAP is >85%, 30 bases-HAP > 80% etc.), and lower cost compared to PAGE or HPLC methods. Oligos produced by the HAP method has such high purity that they can be directly used for any downstream experiments such as PCR, DNA Sequencing, Gene Synthesis, and Mutagenesis.
We can synthesis oligos up to 130 bases in length. Due to manufacturing restrictions we only offer synthesis under PAGE or HPLC purification for oligos ≥60 bases. For our desalted and HAP purification method, there is a size restriction for only oligos between 11-59 bases.
We have a large variety of common modifications. You can find our full on our oligo modifications page. If you cannot find the modification you’re interested in, don’t hesitate to contact us at firstname.lastname@example.org to enquire. Note that some of our modifications may have alternate names as some modification names are proprietary, therefore we are not allowed to list them on our website.
On average it takes about:
2 business days for standard oligos (Desalt and HAP).
+2-3 days for PAGE or HPLC purification.
+1-2 days for longer oligos (longer than 60 bases)
*Turnaround times will vary on current throughput, volumes and complexity of the order.
Oligos are lyophilized in microcentrifuge tubes or lyophilized in 96-well plates for automated operations. Note: A minimum of 48 oligos must be ordered for the 96-well Plate format.
To reconstitute: Centrifuge the tube for a few seconds to collect the DNA in the bottom of the tube. Carefully open, add an appropriate volume of buffer of choice, close, re-hydrate for two minutes and vortex for 15 seconds (common buffers are Tris or TE, TE typically used for stability / long term storage). It is recommended for the oligos to be reconstituted to concentrations > 10µM and stored at -20°C.
Storage at -20°C:
The stability of the lyophilized (dry) and reconstituted material (including TE and and nuclease-free water) is approximately 2 years
Storage at at 4°C:
The stability of the lyophilized (dry) and reconstituted material (including TE and and nuclease-free water) is approximately 1 year
Storage at Room Temperature:
The lyophilized (dry) and TE reconstituted oligos are stable for approx. 6 months, while oligos reconstituted in nuclease-free water would be stable for less than 1 month
– Oligos are more stable when reconstituted in TE buffer rather than in their lyophilized (dry) form. This is because of the stabilization effect contributed by the Tris (maintains pH) and EDTA (inhibits nucleases)
– Stability varies with modified oligos as the different attachments, linkers, etc. alter the stability of the oligos
– Avoid UV light exposure to minimize photodegradation
For Oligo Orders, kindly provide the following information:
A unique oligo name
Sequence in 5′ to 3′
Desired final yield (in OD)
Desired purification (Desalt, HAP, HAP+MS, PAGE, HPLC or HPLC-CE)
Modifications (if any) in their respective location
You may also contact us directly at +65 6954 2519 for any assistance.