Chromatin-Immunoprecipitation Sequencing
Chromatin Immunoprecipitation sequencing (ChIP-Seq) is a technique which combines chromatin immunoprecipitation (ChIP) with NGS for identifying Genome-wide DNA binding sites for transcription factors and other proteins. It could be used to determine how transcription factors and other chromatin-related proteins affect phenotypic mechanisms.
Determining how proteins interact with DNA to regulate gene expression is essential to investigate biological processes and disease states. ChIP-sequencing has played important roles in applications, including studies on gene regulation, transcription complex assembly, DNA repair, histone modification, developmental mechanisms, disease progression and modifications.
Our highly experienced expert team and strict quality control following every procedure ensure the comprehensive and accurate results.
For more information and quotation, kindly contact us at NGS@biobasic-asia.com
Bioinformatics Analysis
- Data Quality Control
- Mapping onto Reference Genome
- Peak calling
- Motif prediction
- Peak annotation (downstream or overlapping gene, TSS)
- Functional analysis of peak-associated genes (Gene Ontology, pathway)
- Visualization of ChIP-Sequencing Data
Advantages
- Captures DNA targets for transcription factors or histone modifications across the entire genome of any organism
- Defines transcription factor binding sites
- Reveals gene regulatory networks in combination with RNA sequencing and methylation analysis
- Utilizing widely accepted software and latest programs for motif prediction, peak annotation, functional analysis and data visualization of ChIP- Sequencing
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