Chromatin Immunoprecipitation sequencing (ChIP-Seq) is a technique which combines chromatin immunoprecipitation (ChIP) with NGS for identifying Genome-wide DNA binding sites for transcription factors and other proteins. It could be used to determine how transcription factors and other chromatin-related proteins affect phenotypic mechanisms.
Determining how proteins interact with DNA to regulate gene expression is essential to investigate biological processes and disease states. ChIP-sequencing has played important roles in applications, including studies on gene regulation, transcription complex assembly, DNA repair, histone modification, developmental mechanisms, disease progression and modifications.
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