Gene Fragment Synthesis

Gene fragments are double-stranded, linear DNA fragments of 100-3,000bp without subcloning into a vector. This is a convenient option due to shorter lead time and lower cost involved.

Gene fragments are widely used in/as:

  1. Assembling of multiple gene fragments to make full-length gene constructs for protein expression and purification

  2. gRNA expression cassettes such as CRISPR/Cas9-based gene editing

  3. Donor constructs

  4. Template for in vitro transcription


Sequence length 100 – 3000 bp
GC content 30 – 68% (Presence of repeats, hairpins, high GC will not be qualified for this service
Protection nucleotide (optional) Kindly indicate if this service is required (This service is NOT included by default).  Please provide details of the preferred protection bases. Otherwise, the default protection bases will be 5’GGAAC-3’GTTCC. 
5’ Phosphorylation (optional) Kindly indicate if this service is required (This service is NOT included by default).


Length (bp) Quantity Lead Time (Business Day)
Price (SGD)
100 – 500 500ng 2 – 5 Please contact our local sales team (
501 – 750
751 – 1000 1000ng 4 – 8
1001 – 1500
1501 – 2250
2251 – 3000


Upon project completion, we will provide:

  • 500 – 1000 ng of lyophilized double stranded DNA

  • QC in Gel electrophoresis image form for size verification (upon request)

For ordering or request of quote, kindly email the completed Gene Synthesis Order Form to and we will get back to you as soon as we can.

You may also contact us directly at +65 6954 2519 for any assistance.

Do kindly provide the following information:

  • Name of genes
  • Length of genes
  • Gene sequences (in .txt/FASTA format)
  • Protection nucleotide (optional)
  • 5′ Phosphorylation (optional)