Excitation & emission wavelengths of fluorophores and recommended quenching groups
*Note: The wavelength data shown is the theoretical value. The wavelength may shift in different degrees in different oligos.
Supporting reporter dyes for multiplex qPCR detection in common quantitative PCR instrument
|
Instrument model |
Dye 1 |
Dye 2 |
Dye 3 |
Dye 4 |
Dye 5 |
|
Agilent MX3000P / 3005P / 4000 |
FAM |
HEX / VIC / JOE / TET |
Cy3 |
Texas red / TEX-615 |
Cy5 |
|
ABI 7300 |
FAM |
HEX / VIC / JOE |
|||
|
ABI 7500 FAST |
FAM |
HEX / VIC / JOE |
Cy3 |
Texas red / TEX-615 |
Cy5 |
|
ABI 7900 HT |
FAM |
HEX / VIC / JOE / TET |
|||
|
ABI QuantStudio Series |
FAM |
HEX / VIC / JOE / TET |
Cy3 |
Texas red / TEX-615 |
Cy5 |
|
ABI StepOne |
FAM |
HEX / VIC / JOE / TET |
|||
|
ABI StepOnePlus |
FAM |
HEX / VIC / JOE |
TAMRA |
||
|
ABI ViiA 7 |
FAM |
HEX / VIC / JOE / TET |
Texas red / TEX-615 |
Cy5 |
|
|
Bio-Rad CFX 96/384 |
FAM |
HEX / VIC / JOE / TET |
Texas red / TEX-615 |
Cy5 |
|
|
Bio-Rad iCycler |
FAM |
HEX / VIC / JOE / TET |
Cy3 |
Texas red / TEX-615 |
Cy5 |
|
Bio-Rad QX200 |
FAM |
HEX / VIC / JOE |
|||
|
Roche LightCycler 96 |
FAM |
HEX / VIC / JOE |
Texas red / TEX-615 |
Cy5 |
|
|
Roche LightCycler 480 |
FAM |
HEX / VIC / JOE / TET |
Texas red / TEX-615 |
Cy5 |
For ordering or request of quote, kindly email the completed Oligo Synthesis Order Form to sg@biobasic-asia.com and we will get back to you as soon as we can.You may also contact us directly at +65 6954 2519 for any assistance.
