Excitation & emission wavelengths of fluorophores and recommended quenching groups

*Note: The wavelength data shown is the theoretical value. The wavelength may shift in different degrees in different oligos.

 

Supporting reporter dyes for multiplex qPCR detection in common quantitative PCR instrument

Instrument model

Dye 1

Dye 2

Dye 3

Dye 4

Dye 5

Agilent MX3000P / 3005P / 4000

FAM

HEX / VIC / JOE / TET

Cy3

Texas red / TEX-615

Cy5

ABI 7300

FAM

HEX / VIC / JOE

ABI 7500 FAST

FAM

HEX / VIC / JOE

Cy3

Texas red / TEX-615

Cy5

ABI 7900 HT

FAM

HEX / VIC / JOE / TET

ABI QuantStudio Series

FAM

HEX / VIC / JOE / TET

Cy3

Texas red / TEX-615

Cy5

ABI StepOne

FAM

HEX / VIC / JOE / TET

ABI StepOnePlus

FAM

HEX / VIC / JOE

TAMRA

ABI ViiA 7

FAM

HEX / VIC / JOE / TET

Texas red / TEX-615

Cy5

Bio-Rad CFX 96/384

FAM

HEX / VIC / JOE / TET

Texas red / TEX-615

Cy5

Bio-Rad iCycler

FAM

HEX / VIC / JOE / TET

Cy3

Texas red / TEX-615

Cy5

Bio-Rad QX200

FAM

HEX / VIC / JOE

Roche LightCycler 96

FAM

HEX / VIC / JOE

Texas red / TEX-615

Cy5

Roche LightCycler 480

FAM

HEX / VIC / JOE / TET

Texas red / TEX-615

Cy5

 

For ordering or request of quote, kindly email the completed Oligo Synthesis Order Form to service@biobasic-asia.com and we will get back to you as soon as we can. You may also contact us directly at +65 6954 2519 for any assistance.