Bio Basic Asia Pacific offers a specialized range of high-purity agarose powder designed for optimal nucleic acid separation and recovery. Our selection includes Low EEO (Electroendosmosis) variants for high-resolution DNA/RNA electrophoresis and Low Melting Point (LMP) agarose for downstream enzymatic applications like cloning and Southern blotting. Each batch is refined to remove charged impurities, ensuring consistent gel strength and thermal stability. Whether you require standard melting-point agarose for routine PCR verification or specialty grades for large-fragment PFGE, our locally-stocked inventory ensures reliable laboratory performance in Singapore.

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Image Product Name Short Description
Agarose A

Agarose A
  • Product Code(s):
    D0012
  • CAS Number:
    [9012-36-6]
  • Grade:
    Biotechnology
  • Storage:
    18 – 25oC
 MORE INFO
Agarose B Low EEO

Agarose B Low EEO
  • Product Code(s):
    AB0014
  • CAS Number:
    [9012-36-6]
  • Grade:
    Biotechnology
  • Storage:
    18 – 25oC
 MORE INFO
Agarose Low Melting Point

Agarose Low Melting Point
  • Product Code(s):
    AB0015
  • CAS Number:
    [9012-36-6]
  • Grade:
    Biotechnology
  • Storage:
    18 – 25oC
 MORE INFO
Showing 3 of 3 Results

Achieve sharp band identification. Pair our specialized agaroses with our Sterile DNA Ladders for precise fragment sizing and high-resolution imaging.

FAQs

1. Why is Low EEO important for high-resolution electrophoresis?

Electroendosmosis (EEO) is a counter-flow of liquid toward the cathode that occurs during electrophoresis due to charged groups in the agarose matrix.1 Low EEO agarose minimizes this resistance, ensuring more predictable migration and sharper resolution, especially for larger nucleic acid fragments.

While gel extraction kits make this possible, standard agarose has a high melting point (> 85 Celcius) that can risk denaturing sensitive DNA.4 Low Melting Point (LMP) agarose is the preferred choice for preparative work as it melts at approximately 65 Celcius, allowing for “in-gel” enzymatic reactions or gentle recovery of intact double-stranded DNA.

Concentration determines pore size: lower concentrations (0.7% – 1.0% provide a larger matrix for high molecular weight DNA (> 85 kb), while higher concentrations (1.5% – 2.5%) offer better sieving for smaller fragments (<1 kb). For extremely small fragments (20-800 bp), a specialized high-resolution agarose at 3%-4% is recommended.

Yes, our molecular biology grade agaroses are strictly tested and certified to have no detectable DNase or RNase activity.15 This purity is essential to prevent sample degradation during separation or downstream extraction.

Slowly sprinkle the powder into room-temperature buffer while stirring constantly before heating.16 Allow the solution to fully rehydrate for a few minutes to prevent “fish-eyes” (undissolved clumps), which can cause anomalous migration and background fluorescence during imaging.