Whenever you look for oligonucleotides (a.k.a. oligos), you often notice their pricing structure is based on a “scale” or “starting scale” in “nmol” quantity. This can be quite misleading as it actually refers to the quantity of “starting material” required to synthesize the oligo and gives you no idea about the final yield of oligo you will be receiving.
(This is like going to a bakery where they ask you to pay for your cake based on the quantity of flower, sugar, etc. they will use, and not the final size of the cake).
Based on the oligo facility and methodology(s) used there, the final yield can vary based on the starting material, with some facilities synthesizing higher yields, while others synthesize lower yields or even mixed yields. In many cases, the final yields are usually not standardized and therefore inconsistent.
We at Bio Basic have modified our oligo synthesis process to allow a simpler and more consistent approach to this pricing structure and final yield deliverable. Because of this we guarantee the final total yield of each oligonucleotide as a FIXED final yield in OD (Optical Density) units.
* = Based on an oligo length of 30 bases.
** = An additional purification cost per oligo for HPLC (non-modified) and HPLC-CE purified oligos.
Remember that the synthesis scale (25 nmol, 100 nmol etc.), refers to the STARTING MATERIAL for oligo synthesis, and NOT the FINAL yield. Oligo length, sequence, GC content and coupling efficiencies alter the final nmol yield. Different purification methods alter the final yield as well. Bio Basic guarantees the final yield of each oligo nucleotide as a fixed yield in OD units.