Cell Cycle Assay Kit

The cell cycle assay kit enables simultaneous analysis of cell cycle distribution and apoptosis by quantifying DNA content in cells. The kit utilizes nucleic acid dyes that specifically bind to nuclear DNA after RNase treatment to eliminate RNA interference, allowing precise measurement of cells in G0/G1, S, and G2/M phases based on their characteristic DNA content levels. Additionally, the assay identifies apoptotic cells through detection of a distinct sub-G1 peak, which results from DNA fragmentation by endogenous nucleases and subsequent loss of DNA fragments from dying cells, making it a comprehensive tool for evaluating both cellular proliferation activity and programmed cell death in a single analysis.