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Description: | The BB Plasmid miniPREP Kit provides a fast, simple, and cost-effective plasmid miniprep method for isolating the plasmid DNA from cultured bacterial cells. The Plasmid miniPREP Kit is based on the alkaline lysis of bacterial cells, followed by the binding of the DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required in this protocol. The high-quality plasmid DNA is then eluted in a small volume of Tris buffer (included in each kit) or water (pH between 7.0 and 8.5). The plasmid DNA purified with our BB Plasmid miniPREP Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation and transformation. The entire procedure can be completed within 20mins. |
Product Code(s): | BMPK00100 |
Sample Type(s): |
Up to 4ml Bacterial cells |
Yield: |
Up to 30µg of Plasmid |
Pack Size(s): |
100 reactions |
Storage: |
Up to 24 months at 18 – 25°C |
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Product Specification | Download |
MSDS | Download |