Description: The BB Plasmid midiPREP Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolating the plasmid DNA from cultured bacterial cells. The Plasmid midiPREP Kit is based on the alkaline lysis of bacterial cells, followed by the binding of the DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required in this protocol. The high-quality plasmid DNA is then eluted in a small volume of Tris buffer (included in each kit) or water (pH between 7.0 and 8.5). The plasmid DNA purified with our BB Plasmid midiPREP Kit is suitable for a variety of routine applications including PCR, restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation and transformation. The entire procedure can be completed within 40mins.
Product Code(s): BMPK00200
Sample Type(s):

Up to 100ml Bacterial cells


Up to 250µg of Plasmid

Pack Size(s):

20 reactions


Up to 24 months at 18 – 25°C