Overview
Bio Basic Asia Pacific has more than a decade of experience in providing quality custom peptide synthesis and also a wide spectrum of modifications (list of modifications) to support your research needs. Generally, the synthesized products ranged from milligram (mg) to kilogram (kg), and with length up to 90 amino acids. These synthesized peptides can come at different purity levels (crude, desalt, >75%, >85%, >90%, >95%, and >98%) according to the requirements of our customers’ projects. The sequence of the synthesized peptides will be quality-assured by ESI mass spectrometry after HPLC purification.
Bio Basic Asia Pacific also offers a wide range of quality pre-made peptides readily available at affordable price. Catalogue of the pre-made peptides is available here.
Workflow
Turnaround Time
| Product | Description | Turnaround Time (Business Day) |
| Standard Polypeptide Synthesis |
|
2 ~ 3weeks |
| N-/C-Terminal Modification | Scale: 1~1000 mg | >2 weeks |
| Customized Amino Acid Modification | ||
|
Fluorescence/Dye Labeling Peptide |
||
| Cyclic Peptide | ||
| Quenched Fluorescent Peptide |
Add-on Services
Optional add-on services such as TFA removal & Solubility Testing, are also available upon request (Price varies with peptide synthesis).
1. TFA Removal (< 1% TFA content) 
Trifluoroacetic acid (TFA) is typically used in peptide synthesis during the process of cleaving peptides from their respective resin, as well as an ion pairing agent in HPLC purifications. Therefore, synthesized peptides are usually in the form of TFA salts (up to 45% TFA content). However, the presence of these TFA-salts can be quite problematic as they are known to cause interferences with the resulting experiment, thereby causing significant discrepancies in data.
2. Solubility Testing 
Peptide solubility can often be a tricky issue. For the first attempt to determine the solubility, we recommend dissolving an aliquot into your choice of buffer, vortexing and/or sonication if necessary.
It is critical that before you order your peptide, determine if it will be soluble in a medium that will be compatible with the experiments you wish to run. After which, we recommend the customer to first attempt to dissolve a small portion of the peptide. This is to prevent reconstituting all the peptide into an inappropriate solvent.
Deliverables
- Final peptide product at customer’s desired scale
- Delivery report
- QC report (inclusive of figures of ESI mass spectrometry and HPLC)
Ordering
For ordering or request of quote, kindly email to sg@biobasic-asia.com the completed Peptide Synthesis Order Form and provide us the following information:
- Peptide sequences (N to C direction)
- Desired quantity
- Purification Method and Purity
- Conjugation or modifications (if any)
You may also contact us directly at +65 6954 2519 for any assistance and enquiries.
Frequently Asked Questions (FAQ)
The synthesized peptide is delivered in the form of a lyophilized powder which comprises the peptide material, counter ion and bound water. Therefore, when you request 1mg of a peptide, by the industry standard it refers to 1mg of lyophilized powder, so the net quantity of your target peptide will be a slightly lesser
HPLC data provides information regarding the purity of the peptide i.e. it will tell you the percentage of your target peptide in relation to the peptide material only (NOT the entire lyophilized powder)
The net peptide content indicates the percentage of the peptide material in relation to the entire lyophilized powder. Typically, the net peptide content is 60-90%. If more accuracy is required, the net peptide content can be obtained by Amino Acid Analysis (AAA) or Elemental Analysis (EA)
AAA requires a smaller quantity of the peptide for analysis and is less accurate and cheaper to perform. EA requires larger quantities of the peptide for analysis and more accurate and its more costly to perform. Generally if the net peptide content is crucial for your research, EA is recommended for your project
We provide both MS and HPLC with all peptide synthesis to ensure the sequence is correct
All peptides are in lyophilized form, which are stable at room temperature. However to ensure a long shelf life please use a freezer. It is recommended to store the lyophilized form at -20°C, or preferably at -70°C for peptides in solution. Please ensure peptides are in sealed containers to minimize peptide degradation.
Crude: Non-sensitive screening assays.
>70%: Non sensitive screening assays, exploratory or relative MS assays.
>80%: Non quantitative binding assays, ELISPOT assays, Polyclonal antibody production.
>90%: Post-translationally modified peptides for polyclonal antibody productions,
receptor-ligand assays.
>95%: Quantitative Mass Spectrometry SRM/MRM assays, monoclonal antibodies, quantitative receptor-ligand assays, enzyme kinetics, cell/tissue culture, phosphorylation studies.
>98%: NMR, X-ray structural studies.
The minimum amount to be ordered is 1 mg. There is no maximum amount.
Please click here for our gene services terms and conditions
For ordering or request of quote, kindly email to sg@biobasic-asia.com the completed Peptide Synthesis Order Form and provide us the following information:
- Peptide sequences (N to C direction)
- Desired quantity
- Purification Method and Purity
- Conjugation or modifications (if any)
You may also contact us directly at +65 6954 2519 for any assistance and enquiries.
REQUEST A QUOTE NOW
