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Details | |
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Description: |
BspQI is categorized as an isoschizomer of SapI, one of the SpeedyCut series of restriction enzymes (RE) but with better stability. The specialized buffer employed optimizes the activity of BspQI, making it unsuitable for use with the universal SpeedyOne buffer. The specialized 10X buffer can be used interchangeably with BsmBI RE (SGRE00100). Notably, this reaction buffer features a recombinant albumin component, significantly enhancing the enzyme’s stability. Belonging to the Type IIS class of RE, BspQI recognizes non-palindromic DNA sequences and performs digestion beyond the recognition site. BspQI is widely applied in experimental techniques such as Golden Gate assembly, rapid cloning, and large-scale plasmid linearization. |
Recognition Site: | 5’…-GCTCTTC(N)1↓…-3′ 3’…-CGAGAAG(N)4↑…-5′ |
Product Code(s): | SGRE00200 |
Pack Content(s): |
BspQI RE – 50μl (500U) 10X BspQI buffer – 1ml |
Storage: |
Stored at -20℃ |
File | Download |
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Product Specification | Download |
MSDS | Download |