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Description: | The BB Plasmid maxiPREP Kit provides a fast, simple, and cost-effective plasmid maxiPREP method for isolating the plasmid DNA from cultured bacterial cells. The BB Plasmid maxiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required in this protocol. High-quality plasmid DNA is eluted in a small volume of Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the BB Plasmid maxiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The BB Plasmid maxiPREP Kit ensures safety to users as it eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, thus minimizing the exposure to and disposal of hazardous materials. The entire procedure can be completed within 40mins. |
Product Code(s): | BMPK00300 |
Sample Type(s): |
Up to 200ml Bacterial cells |
Yield: |
Up to 850µg of Plasmid |
Pack Size(s): |
10 reactions |
Storage: |
Up to 24 months at 18 – 25°C |
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Product Specification | Download |
MSDS | Download |