Bio Basic Asia Pacific Pte Ltd

BB FasTaq DNA Polymerase

Catalog Number: BPR00400
Availability: In Stock
SGD 29.00

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Details
Description: BB FasTaq DNA Polymerase is purified from E. coli. expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and a 5′ → 3′ exonuclease activity but without a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94kDa. BB FasTaq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. The BB FasTaq Polymerase is recommended for use in routine PCR reactions. The buffer system is optimized for high specificity and guarantees minimal by-product formation. We supplied BB FasTaq DNA Polymerase with appropriate buffers. Usually, 1-1.5U of FasTaq DNA Polymerase is used in 50µl of the reaction mix. Higher FasTaq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of FasTaq DNA Polymerase (2-3U) may be necessary to obtain a better yield of amplification products
Product Code(s): BPR00400
Content(s):

BB FasTaq DNA Polymerase –  100μl x 1 vial

10X PCR buffer  –  1,250μl x 2 vial

Storage:

Up to 12 months at -20°C