Microbial Whole Genome Sequencing

Microbial whole genome sequencing is important for precise microbial identification, the generation of complete reference genomes (de novo sequencing), comparative genomic studies (re-sequencing), and genomic exploitation.

Comparative genomic studies can identify individual genetic variations and large scale structural variations within a population for which a reference genome is available. Evolutionary characteristics and phylogenetic relationships can be hence inferred.

Microbial whole genome sequencing provides the possibility of gene finding and annotation. After multiple genes are explained, novel biochemical pathways that may be beneficial for medicine and biotechnology will likely be identified.

Bioinformatics Analysis

For Requencing

  • Data quality control
  • Alignment with reference
  • SNP/InDel calling, annotation and statistics
  • SV calling, annotation, and statistics

(More analysis upon request)

For de novo

  • Data Quality Control
  • Genome advanced assemble
  • Genome survey: genome size, GC distribution, repeat rate
  • Genome component analysis: gene structure prediction, repeat sequences, non-coding RNAs
  • Gene function annotation

(More analysis upon request)

Advantages

Image source: Shi, C., Liu, Y., Huang, H., Xia, E.-H., Zhang, H.-B., & Gao, L.-Z.. (2015). Circular plot of transcriptome reads mapping of the C. sinensis var. assamica chloroplast genome. (Version 1). PLOS ONE. https://doi.org/10.1371/journal.pone.0059620.g005

  • Broad applications: de novosequencing, gene annotations, comparative genomic studies,
    evolutionary studies of micro-organisms, etc.
  • Drug discovery and development: assess the contribution of DNA on pathogenesis and understand the role of mobile elements in drug resistance and transmission
  • Cost-effective
  • Time-efficient

Analysis Workflow

For more information, feel free to contact us via NGS@biobasic-Asia.com or +65 6928 9042.