Long-Read Metagenomic Sequencing

Metagenomics sequencing is the way to do genomic analysis of microbial DNA from environmental communities.

It enables the population analysis of unculturable or previously unknown microbes and allows researchers to  comprehensively sample all genes in all organisms present in a given complex sample.

Now Bio Basic Asia Pacific provides long-read metagenomics sequencing service on PacBio SMRT System in a culture-free method.

Long-read metagenomics sequencing help scientists generating new insights into the function and pathway facets of the microbiome, trying to understand and elucidate the relations between microbes and its habitat/host.

This is a powerful way to obtain microbial genomes with the highest accuracy and reliability.

Principle of SMRT sequencing:
Fluorescence from a dye-linked nucleotide is detected during DNA extension.
Different emission from the various dye allow identification of the incorpated base.
Image source: https://www.sciencedirect.com/science/article/pii/S1672022915001345

Bioinformatics Analysis

  • Gene prediction
  • Non-redundant Gene Catalog
  • Functional annotation: KEGG, eggNOG, CAZy.
  • Taxonomic annotation
  • Pathway, Heatmap, PCA, Clustering

(More upon request)

Advantages

  • Longest average read lengths, with~50% of reads longer than 50kb, which exceeds the size of repetitive elements in the average bacterial genome.
  • Identify full-length genes within a microbial community using long-read metagenomic profiling with no assembly required
  • No DNA amplification.
  • Highest consensus accuracy, low sequencing-context bias

Analysis Workflow

For more information, feel free to contact us via NGS@biobasic-asia.com or +65 6928 9042.