Full-Length 16S/18S/ITS Amplicon Sequencing

Over the past 10 years, 16S/18S/ITS rRNA gene sequencing is the best tool to study bacterial and fungal taxonomy, and molecular phylogeny.

By taking advantage of PacBio SMRT long reads sequencing technology, Bio Basic Asia Pacific can provide full length 16S/18S/ITS rRNA sequencing service to help your research.

Take 16S rRNA as an example, the average read length of the PacBio SMRT sequences is 10~18 K, which can cover all variable areas of16S rRNA (the total length is around 1,500 bp).

Meanwhile, circular consensus sequencing (CCS) produces high intra-molecular consensus accuracy for full-length 16S rDNA to achieve accurate to “species” classification and identification.

18S rRNA (total length 1,500-2,000 bp) and ITS (about 400-900 bp) are also able to be performed by full-length sequencing for microbiome analysis.

Bioinformatics Analysis

  • OTUs clustering and filtering
  • OTUs analysis and species annotation
  • PCA, Venn diagram. A rank curve will be generated based on OTU abundance
  • Alpha diversity, Beta diversity, Meta-analysis
  • Multi-variate statistical analysis

(More upon request)

Advantages

Strain-level identification by the full length of amplicons

Analysis Workflow

For more information, feel free to contact us via NGS@biobasic-Asia.com or +65 6928 9042.