De Novo Sequencing

Denovo sequencing refers to sequencing a novel genome where there is no reference sequence available for alignment. The coverage quality of denovo assembly depends on the size and continuity of the contigs.

De novo sequencing generates the first genome map for a species, thus providing a valuable reference sequence for re-sequencing.  Oxford Nanopore and PacBio sequencing systems enable a faster and more accurate characterization of any species at the nucleotide level.

Bio Basic Asia Pacific combines both Illumina HiSeq and PacBio systems to provide a fast and accurate de novo sequencing and bioinformatics analysis for any species.

Our highly experienced expert team executes quality management, following every procedure to ensure reliable results.

Bioinformatics Analysis

  • Data Quality Control
  • Genome Preliminary Assemblies
  • Genome Annotation

(More analysis upon request)

Advantages

  • Generates accurate reference sequences, even for complex or polyploid genomes
  • Provides useful information for mapping genomes of novel organisms or finishing genomes of known organisms
  • Identifies structural variants and complex rearrangements, such as deletions, inversions, or translocations

Sequencing Platforms 

HiSeq 4000/ HiSeq X Ten/ NovaSeq 6000
PacBio RSII/ Sequel

Analysis Workflow

For more information, feel free to contact us via NGS@biobasic-asia.com or +65 6928 9042.