Chromatin Immunoprecipitation Sequencing

Chromatin Immunoprecipitation sequencing (ChIP-Seq) is a technique which combines chromatin immunoprecipitation (ChIP) with NGS for identifying Genome-wide DNA binding sites for transcription factors and other proteins.

It could be used to determine how transcription factors and other chromatin-related proteins affect phenotypic mechanisms.

Determining how proteins interact with DNA to regulate gene expression is essential to investigate biological processes and disease states.

ChIP-Sequencing  has played important roles in applications, including studies on gene regulation, transcription complex assembly, DNA repair, histone modification,

developmental mechanisms, disease progression and modifications.

Bioinformatics Analysis

  • Data Quality Control
  • Mapping onto Reference Genome
  • Peak calling
  • Motif prediction
  • Peak annotation (downstream or overlapping gene, TSS)
  • Functional analysis of peak-associated genes (Gene Ontology, pathway)
  • Visualization of ChIP-Sequencing Data

Advantages

  • Captures DNA targets for transcription factors or histone modifications across the entire genome of
    any organism
  • Defines transcription factor binding sites
  • Reveals gene regulatory networks in combination with RNA sequencing and methylation analysis
  • Utilizing widely accepted software and latest programs for motif prediction, peak annotation,
    functional analysis and data visualization of ChIP- Sequencing

Analysis Workflow

For more information, feel free to contact us via NGS@biobasic-asia.com or +65 6928 9042.