Chromatin Immunoprecipitation sequencing (ChIP-Seq) is a technique which combines chromatin immunoprecipitation (ChIP) with NGS for identifying Genome-wide DNA binding sites for transcription factors and other proteins.
It could be used to determine how transcription factors and other chromatin-related proteins affect phenotypic mechanisms.
Determining how proteins interact with DNA to regulate gene expression is essential to investigate biological processes and disease states.
ChIP-Sequencing has played important roles in applications, including studies on gene regulation, transcription complex assembly, DNA repair, histone modification,
developmental mechanisms, disease progression and modifications.
Bioinformatics Analysis
- Data Quality Control
- Mapping onto Reference Genome
- Peak calling
- Motif prediction
- Peak annotation (downstream or overlapping gene, TSS)
- Functional analysis of peak-associated genes (Gene Ontology, pathway)
- Visualization of ChIP-Sequencing Data
Advantages
- Captures DNA targets for transcription factors or histone modifications across the entire genome of
any organism - Defines transcription factor binding sites
- Reveals gene regulatory networks in combination with RNA sequencing and methylation analysis
- Utilizing widely accepted software and latest programs for motif prediction, peak annotation,
functional analysis and data visualization of ChIP- Sequencing
Analysis Workflow
For more information, feel free to contact us via NGS@biobasic-asia.com or +65 6928 9042.